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degradation of cholesterol pdf

(When this article is cited)Tj <> <> -10.78099 0 Td endobj <> 218 0 obj <> endobj degradation of cholesterol 1. endobj endobj <> [411 0 R 412 0 R 413 0 R 414 0 R 231 0 R] 215 0 obj [539 0 R] ( )Tj -7.88899 0 Td [420 0 R 421 0 R 422 0 R 423 0 R 233 0 R] 504 0 obj ( by guest on December 27, 2020)Tj 2020-12-27T13:19:36-08:00 ARTICLE Insig-mediated degradation of HMG CoA reductase stimulated by lanosterol, an intermediate in the synthesis of cholesterol Bao-Liang Song,1 Norman B. Javitt,2 and Russell A. DeBose-Boyd1,* 1Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390 2Department of Pediatrics and Medicine, New York University School of Medicine, New York, … <> 249 0 obj endobj 448 0 obj 2003-02-19T11:44:14+05:30 [225 0 R 226 0 R 227 0 R 228 0 R 229 0 R 238 0 R 239 0 R 240 0 R 241 0 R 242 0 R 243 0 R 244 0 R 245 0 R 246 0 R 247 0 R 248 0 R 249 0 R] S. C. §1734solely toindicate Downloaded by guest on … <>stream <> (\240)Tj [321 0 R 322 0 R 323 0 R 324 0 R 325 0 R 326 0 R 327 0 R 328 0 R 329 0 R 330 0 R 331 0 R 332 0 R 333 0 R 334 0 R 335 0 R 336 0 R 337 0 R 338 0 R] 459 0 obj ( )Tj 217 0 obj 23 cells used as the inoculum in UHT milk rather than in an\ud artificial medium, prior to determination of cholesterol degradation.\ud The cholesterol degrading system of R. equi No. BT In endoplasmic reticulum (ER) membranes, SREBPs form complexes with Scap, a cholesterol-sensing membrane protein. endobj 2004). endobj (tml#ref-list-1)Tj 207 0 obj S <> Acrobat 4.0 Import Plug-in for Windows Get a printable copy (PDF file) of the complete article (2.5M), or click on a page image below to browse page by page. endobj /T1_3 1 Tf 10 0 0 10 89 343.99997 Tm <> <> endobj 496 0 obj 200 0 obj When cholesterol levels are low, Scap escorts SREBPs from the ER to the Golgi where proteolytic cleavage releases the transcription factor … H��WkS�J��_��I�-���o�{/�6��wkK���W�P�_�3�L�r�&U�T==�8}�̟�yNx��? %PDF-1.5 %���� 10 0 0 10 154.01996 492 Tm endobj 89 468 m <> 0 0 1 rg 455 0 obj 1.P.47 Degradation of pyrene-labeled cholesterol esters by lysosomal lipase in vitro and in vivo. -18.61797 0 Td (DEGRADATION OF CHOLESTEROL)Tj <>/Metadata 502 0 R/Pages 2 0 R/StructTreeRoot 3 0 R/Type/Catalog>> 214 0 obj 10 0 0 10 89 413.99994 Tm endobj 0 g 503 0 obj 468 0 obj <> /T1_0 1 Tf It was suggested that hypolipidemic activity of okra was mediated most likely by upregulation of cholesterol degradation through CYP7A1 and by inhibition of lipogenesis through SREBP1c and FAS. BT 492 0 obj (alerts)Tj 150 0 obj endobj application/pdf [492 0 R 493 0 R 494 0 R 495 0 R 496 0 R 497 0 R 498 0 R] endobj endobj 536 0 obj 497 0 obj endobj <> endobj endobj endobj Cholesterol-Mediated Degradation of 7-Dehydrocholesterol Reductase Switches the Balance from Cholesterol to Vitamin D Synthesis endobj <>/ProcSet[/PDF/Text]>>/Type/Page>> endobj Introduction to Cholesterol Metabolism. endobj endobj 502 0 obj Brown and C. … (Click here)Tj Substrate Concentration. endobj 19.67698 1 Td 470 0 obj <> Brain cholesterol is catabolized to 24S-hydroxycholesterol (24S-OHC) by the neuron-specific endobj 202 0 obj 348 0 obj 467 0 obj <> <> '����~���Z�`Ϩ��,��MSdF��$i��SI9�GM@\}/���coz�RjO]b�Đt�@a�[Um����P�O���O�:D��iH ����Lq�wI���b��AY��S+@`v#%@��q�� ��79R�!�5F��ȉgy�� �xȃI9^�H,�)8>/�%*K�=�Eq2�Y����d����Ve8�������^T��(�hŇ��\��/e���o��Zy. <>/MediaBox[0 0 609.84 792.12]/Parent 534 0 R/Resources<>/Font<>/ProcSet[/PDF/Text]>>/StructParents 21/Tabs/S/Type/Page>> thermal degradation of cholesterol were reduced greatly after incorporation of quercetin [17]. [506 0 R] (1954, 206:511-523. <> endobj endobj ET <> -0.22452 1.00001 Td <> )Tj 402 0 obj <> This paper studied the degradation of cholesterol and three plant sterols during a 360 min heating treatment (180ºC). endobj Phytosterols and cholesterol share a similar chemical structure, and undergo oxidation during food processing and storage. [307 0 R 308 0 R 309 0 R 310 0 R 311 0 R 312 0 R 313 0 R 314 0 R 315 0 R 316 0 R 317 0 R 318 0 R 319 0 R 320 0 R] Cellular Cholesterol Regulates Ubiquitination and Degradation of the Cholesterol Export Proteins ABCA1 and ABCG1* @article{Hsieh2014CellularCR, title={Cellular Cholesterol Regulates Ubiquitination and Degradation of the Cholesterol Export Proteins ABCA1 and ABCG1*}, author={V. Hsieh and Mi-Jurng Kim and I. Gelissen and A. <> endobj /T1_0 1 Tf 206 0 obj ET 312 468 l Under physiological conditions, approximately 300–400 mg of cholesterol are disposed of in the liver daily, through this pathway [3]. [438 0 R 439 0 R 440 0 R 441 0 R 442 0 R 236 0 R] L-a-distearoyl phosphatidylcholine; CH,cholesterol. 10 0 0 10 89 278.99988 Tm 447 0 obj 201 0 obj 0 g 2 0 obj endobj <> endobj Cholesterol accelerated the proteasomal degradation of SM which required the N-terminal domain, partially conserved in vertebrates but not in lower organisms. Normal cholesterol levels are strictly maintained by de novo synthesis and degradation and cells can sense their level of cholesterol through the membrane-bound sterol regulatory element-binding protein (SREBP) (Brown and Goldstein, 1997). /T1_3 1 Tf (When a correction for this article is posted)Tj /T1_3 1 Tf endobj <> 10 0 0 10 109 368.99994 Tm The bile acids are synthesized in the liver from cholesterol. Links to PubMed are also available for Selected References. BT <> [291 0 R 292 0 R 293 0 R 294 0 R 295 0 R 296 0 R 297 0 R 298 0 R 299 0 R 300 0 R 301 0 R 302 0 R 303 0 R 304 0 R 305 0 R 306 0 R] 210 0 obj 390 0 obj <> -3.985 1 Td <> F���L�&֖��|����nή� D�w�p�2�1ѥ�m:�ݤ�e��7A��&�>4L��x�a��:ǹ�=*�x3#�NJ�]%r%��c�?7��������6�u�Ij~�| Υu�;�]�H���Y>�Pq-1���'�z�,7λ5�<8�I{@�"F~�$C��ιo#S3���R�{[s�p z\��k��x����3"l�$�as�=����E`$Ȩ�7EՖ �6��W��>�/a�ĕ��KR� (Christian B. Anfinsen)Tj 458 0 obj ��e����8h��c����&��@�5�����y�;,�toQ�������q��v�8��[�&�,2��)�)�F6R�ֲ�����i�`0�]��2�����)+���т���� 5.49997 1 Td 454 0 obj 487 0 obj 315 0 obj [369 0 R 370 0 R 371 0 R 372 0 R 373 0 R 374 0 R 375 0 R 376 0 R 377 0 R 378 0 R 379 0 R 380 0 R 381 0 R 382 0 R 383 0 R] Examination of cholesterol utilization dur-ing mycobacterial growth using the coupled cholesterol- 468 0 obj Cellular cholesterol levels are also modulated by a cycle of cholesterol esterification by acyl- CoA : cholesterol acyltransferase (ACAT) and hydrolysis of the cholesteryl esters, and by cholesterol metabolism to … endobj <>/ProcSet[/PDF/Text/ImageB]/XObject<>>>/Rotate 0/Type/Page>> 514 DEGRADATION OF CHOLESTEROL diluted to 10 ml. 209 0 obj [396 0 R 397 0 R 398 0 R 447 0 R 448 0 R 448 0 R 448 0 R 449 0 R 450 0 R 450 0 R 450 0 R 450 0 R 451 0 R 452 0 R 452 0 R 452 0 R 452 0 R 452 0 R 452 0 R 452 0 R 453 0 R 454 0 R 454 0 R 454 0 R 454 0 R 454 0 R 454 0 R 455 0 R 456 0 R 457 0 R 458 0 R 459 0 R 460 0 R 461 0 R 462 0 R 463 0 R 464 0 R 465 0 R 466 0 R 467 0 R 468 0 R 469 0 R 470 0 R 471 0 R 472 0 R 473 0 R 474 0 R 475 0 R 400 0 R 401 0 R 402 0 R] endstream 475 0 obj 10 0 0 10 120.11996 399 Tm Cholesterol accumulation and degradation by mycobacteria Three different experimental approaches were used to de-termine cholesterol utilization by mycobacteria, specifically colorimetric determination of cholesterol levels, TLC, and HPLC analysis. Cholesterol increases the risk of aggressive pros- 449 0 obj 408 0 obj ACKNOWLEDGEMENTS The se last p ages of a PhD thesis a re usually the most widely re ad pages of the entire publication. <> 3 0 obj 282 0 obj 485 0 obj uuid:f2996e4c-1dd1-11b2-0a00-ad09271d5700 [424 0 R 425 0 R 426 0 R 427 0 R 428 0 R 429 0 R 430 0 R 234 0 R] 221 0 obj <> 203 0 obj The isolated microorganisms were grown in the 2016-02-09T17:57:47+11:00 brown lab Thisarticle mustthereforebeherebymarked"ad-vertisement"inaccordancewith 18U. <> endobj -0.906 0 Td In this report, our objective was to develop an integral mathematic model for studying the thermal degradation or oxidation of quercetin in the presence and absence of cholesterol. 11.68699 1.00001 Td 205 0 obj -7.55399 0 Td endobj Free fulltext PDF articles from hundreds of disciplines, all in one place Novel Inhibitors of Cholesterol Degradation in Mycobacterium tuberculosis Reveal How the Bacterium’s Metabolism Is Constrained by the Intracellular Environment (pdf) | Paperity <> 491 0 obj <> 7- /T1_0 1 Tf DOI: 10.1074/jbc.M113.515890 Corpus ID: 10279237. endobj (\240 )Tj 219 0 obj 498 0 obj endobj 397 0 obj -19.67698 0 Td 0 1 TD 398 0 obj During cholesterol degradation, three further intermediate compounds were identified by their characteristic mass spectrum, namely, 26-hydroxycholest-4-en-3-one (m/z 401), 26-hydroxycholesta-1,4-dien-3-one (m/z 399), and cholest-4-en-3-one-26-oic acid (m/z 415) in parallel to cholest-4-en-3-one. [268 0 R 269 0 R 270 0 R 271 0 R 272 0 R 273 0 R 274 0 R] BT <> /T1_3 1 Tf [443 0 R 444 0 R 445 0 R 446 0 R 237 0 R] [259 0 R 259 0 R 259 0 R 259 0 R 259 0 R 259 0 R 260 0 R 261 0 R 262 0 R 263 0 R 264 0 R 265 0 R 266 0 R 267 0 R] endobj endobj -0.66701 1 Td FK�`�����I.4�3`>��nX�s�ƎQ&��"X.8�V'�?5���`d������"k�^z�&��[Z��0By ��Gc�HP9���AV[�qҲ�MN�gMC��7�����j�4o�|���+ �E� �����ug��!���G������~^���C:��� H��W[o۸~ϯࣵX˼SZ���Ӄ؞xq���M'���:��ߡ$�#�T�8yHb�3�����Ofﶻ���厼y3{��-.o�+r1;��v��:�?�Wd��by�^�n7k�����{r2�������l>����J2�>Q��Z�rY���ɄI�����X�5��ȵ�d�DN��������,�O���L�����l����3B����e�h�rE�g'�y&sJ�d�.Sdr�M5|��$�O��C&�ʐ�yFseJ�@�:��8�0P��sm���7y�� G���]�M�G�M+�'@��L7�R��Fm�.&����u�Z�n�R����-8�����QXeL���hP�M�`e����4�p���_@�)�������M�q�W�,S�P��L~D�d*�� �QQZq@�Oƨ]�eSP�8{�Ycq6�5������l����^�և %PDF-1.4 %���� (http://www.jbc.org/)Tj endstream endobj 0 0 1 rg The formation and further degradation of SOPs was also analyzed by GC-MS. (STUDIES ON THE MICROBIOLOGICAL)Tj well documented (7, 9, 19, 27, 32, 33), but the degradation of cholesterol by a member of the genus Gordonia was reported only recently (8). ET <> Chem.\240)Tj -5.49997 0 Td BT 0 0 1 rg Degradation of Cationized Low Density Lipoprotein and Regulation of Cholesterol Metabolism in Homozygous Familial Hypercholesterolemia Fibroblasts Cholesterol and phytosterols can suffer oxidation under heating conditions to give Sterol Oxidation Products (SOPs), known by their toxic effects. 0 0 1 rg <> With dialyzed preparations, it was possible to follow cholestenone for- mation from cholesterol by measuring the increase in absorption at 240 rnp in 90 per cent ethanol extracts. 401 0 obj endobj 472 0 obj Degradation to primary bile acids represents the most relevant mechanism of irreversible elimination of cholesterol from the body, thus playing a key role in hepatic and systemic cholesterol homeostasis. 462 0 obj 535 0 obj 0 g <> <> 17 0 obj ET Keywords: 7-dehydrocholesterol, cholesterol, DHCR7, degradation, vitamin D ABSTRACT . endobj 213 0 obj 0 g Maximum cholesterol degradation by the isolates occurred in the cholesterol medium (Medium-A), where cholesterol (1 g/L) was supplemented with as a sole carbon source for 6 d. Approx, 65-80% of the cholesterol was degraded by the isolates (Table 2). The studied strains degrade cholesterol by different mechanisms and may suggest a new possibility for the mechanism underlying cholesterol degradation by LAB. Cholesterol (from the Ancient Greek chole-() and stereos (solid), followed by the chemical suffix-ol for an alcohol) is an organic molecule.It is a sterol (or modified steroid), a type of lipid. HepG2 cells and HuH-7 cells both originated from a human hepatoma and endogenously express ABCA1. endobj endobj endobj endobj The maximum cholesterol degradation (about 90%) was obtained on the third day. In these preparations cholesterol degradation was further enhanced by\ud growth of R. equi No. 4-cholesten-3-one was detected as a degradation product of cholesterol by Leuconostoc mesenteroides GMK03. 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